Biotin-conjugated anti-MMAE mAb

Background

Monomethyl auristatin E (MMAE, vedotin) is a very potent antimitotic agent that inhibits cell division by

blocking the polymerisation of tubulin. The family of auristatins are synthetic analogues of the antineoplastic

natural product Dolastatin 10, ultrapotent cytotoxic microtubule inhibitors that are clinically used as payloads in

antibody-drug conjugates. Antibody-drug conjugates enhance the antitumor effects of antibodies and reduce

adverse systemic effects of highly potent cytotoxic agents. MMAE is actually desmethyl-auristatin E; that is, the

N-terminal amino group has only one methyl substituent instead of two as in auristatin E itself.

Biotin-anti-MMAE-antibody is a conjugation product of Biotin and anti-MMAE-antibody. It can be used in PK,

PD analysis and ELISA.

Specificity: Specially recognizes the Monomethyl Auristain E (MMAE)

Source: Mouse, Monoclonal Clone #04

Isotype: IgG1

Conjugate: Biotin

Concentration: 1.0 mg/ml

Tested applications: ELISA

Recommended dilution: 1:1000 - 1:5000(optimal dilutions should be determined by the end use.)

Storage Buffer: PBS Buffer, pH7.4

Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2

years from date of receipt. Aliquot to avoid repeated freezing and thawing.

* Please note: FOR RESEARCH USE ONLY