Background
Monomethyl auristatin E (MMAE, vedotin) is a very potent antimitotic agent that inhibits cell division by blocking
the polymerisation of tubulin. The family of auristatins are synthetic analogues of the antineoplastic natural
product Dolastatin 10, ultrapotent cytotoxic microtubule inhibitors that are clinically used as payloads in
antibody-drug conjugates. Antibody-drug conjugates enhance the antitumor effects of antibodies and reduce
adverse systemic effects of highly potent cytotoxic agents. MMAE is actually desmethyl-auristatin E; that is, the
N-terminal amino group has only one methyl substituent instead of two as in auristatin E itself.
Immunogen: MMAE-KLH
Specificity: Specifically recognizes the target-MMAE
Source: Mouse, Monoclonal Clone #04
Isotype: IgG1
Conjugate: Unconjugated
Concentration: 1.0 mg/ml
Purity: > 90% as determined by SDS-PAGE
Tested applications: ELISA
Recommended dilution: 1:1000 - 1:5000 (optimal dilutions should be determined by the end use.)
Storage Buffer: PBS Buffer, pH7.4
Storage: Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Stable for 2
years from date of receipt. Aliquot to avoid repeated freezing and thawing
* Please note: FOR RESEARCH USE ONLY
